Talk Title: Using ultra high-throughput single cell sequencing to understanding cellular heterogeneity
The transcriptome is a key determinant of the phenotype of a cell and regulates the identity and fate of individual cells. Much of what we know about the structure and function of the transcriptome comes from studies averaging measurements over large populations of cells, many of which are functionally heterogeneous. Such studies conceal the variability between cells and so prevent us from determining the nature of heterogeneity at the molecular level as a basis for understanding biological complexity. Cell-to-cell differences in any tissue or cell culture are an essential feature of their biological state and function. This talk will cover recent work from my lab in generating and analyzing ultra high-throughput single cell RNA sequence data from cell cultures, and both solid and circulating human tissues. I will discuss some of the technical and computational challenges, and highlight possibilities for the rapid clinical utility of single cell sequencing technology.
Dr Joseph Powell, Senior Research Fellow, Institute for Molecular Bioscience and Affiliate Senior Research Fellow, Queensland Brain Institute
Joseph Powell is the head of the Single Cell and Computational Genomics Lab at the University of Queensland’s Institute for Molecular Bioscience and an NHMRC Career Development Fellow. His research involves the use of large-scale transcriptomic and DNA sequence data from both bulk tissues and single cells, focusing on understanding the genetic mechanisms by which heritable variants contribute to disease susceptibility at a cellular level, and ultimately achieve therapeutic and diagnostic outcomes. His group consists of both a high-throughput single cell sequencing laboratory and a core of computational genomics expertise.