Sheep Functional annotation reveals proximal regulatory elements contributed to the evolution of modern breeds

Domestication fundamentally reshaped animal morphology, physiology and behaviour, offering the opportunity to investigate the molecular processes driving evolutionary change. Here we assess sheep domestication and artificial selection by comparing genome sequence from 43 modern breeds (Ovis aries) and their Asian mouflon ancestor (O. orientalis) to identify selection sweeps. Next, we provide a comparative functional annotation of the sheep genome, validated using experimental ChIP-Seq of sheep tissue. Using these annotations, we evaluate the impact of selection and domestication on regulatory sequences and find that sweeps are significantly enriched for protein coding genes, proximal regulatory elements of genes and genome features associated with active transcription. Finally, we find individual sites displaying strong allele frequency divergence are enriched for the same regulatory features. Our data demonstrate that remodelling of gene expression is likely to have been one of the evolutionary forces that drove phenotypic diversification of this common livestock species.

Marina Naval Sanchez

Marina Naval Sanchez

CSIRO

Marina Naval Sanchez received a BS degree in Agri-food from Universitat de Lleida, a Msc in Agriculture Engineering, majors in animal biotechnology, from Universitat de Lleida, and a MSc in Applied bioinformatics from Cranfield University, UK. She completed a PhD from KULeuven, Belgium, under the mentorship of Professor Stein Aerts. From 2015 she moved to Australia as an OCE PostDotctoral fellow to work at CSIRO, Brisbane, with Dr. James Kijas and currently with Dr. Toni Reverter. Her main research interests include genomics, transcriptomics, populations and evolutionary genomics and gene regulatory networks, focusing on livestock species.

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