Detailed characterization of the cell types in human tissues requires scalable approaches to examine multiple aspects of the molecular states as well as how such states are regulated or maintained. We have established a number of experimental and computational approaches to characterize the transcriptional control and outcomes at scale in single cells. We have applied snDrop-seq and scTHSseq to human fetal and adult brain samples to identify cell types and regulatory elements in the developing and adult brains. We also experimentally linked transcription and chromatin accessibility in the same single cells, which provides an unprecedented resolution for constructing the transcriptomic and epigenetic landscapes of cell types in human brain, kidney and lung.
Professor and Chair, Department of Bioengineering, University of California, San Diego
Dr. Kun Zhang is a Professor and Department Chair of Bioengineering at the University of California San Diego. His group develops technologies related to single-cell analyses of genome, epigenome and transcriptome, as well as single-cell imaging and lineage tracing. These novel technologies are applied to stem cell fate conversion, human brain mapping and human disease studies. He is currently leading multiple NIH center projects on creating the 3D single-cell maps for multiple human organs, including brain, lung, kidney and bladder.