Gastrointestinal microbiota and immune cells interact closely and display regional specificity; however, little is known about how these communities differ with location. In this presentation I will talk about how single-cell RNA sequencing and 16S sequencing has been leveraged to simultaneously assess microbiota and single immune cells across the healthy, adult human colon to delineate colonic immune niches at steady state. This allowed for the description of increasing B cell accumulation, clonal expansion and mutational frequency from the cecum to the sigmoid colon and link this to the increasing number of reactive bacterial species (James et al., Nat. Immunol., 2020). This work provides a reference map of the cellular and microbial landscape of the healthy gut that can inform on changes in these populations underpinning disease.
Team Leader, Garvan Institute of Medical Research
Spinak Fellow supported by an NHMRC Investigator Grant
Dr Kylie James applies single-cell methods to understand cellular landscapes of the gastrointestinal tract in response to environmental challenges and disease (James KR, et al., Nat. Immunol., 2020, GutCellAtlas.org). Kylie completed her PhD at QIMR Berghofer Medical Research Institute under the supervision of Dr Ashraful Haque. She moved to the UK to complete a postdoctoral position at the Wellcome Sanger Institute with Dr Sarah Teichmann and Junior Research Fellowship with Christ’s College, University of Cambridge. She has recently moved to the Garvan Institute of Medical research as a Team Leader and Spinak Fellow supported by an NHMRC Investigator Grant.