Dr Xiaotao Jiang

WORKSHOP: A beginner’s guidance on 16S amplicon microbiome analysis

This workshop will cover a real demo example using 16S amplicon sequencing to investigate the microbiome in a comparative study design. Students will be able to learn the complete analysis from the raw sequencing data. Quality control with dada2, taxonomy annotation, alpha and beta diversity analysis with QIIME2 and differential abundant analysis will be covered.

Keywords: 16S amplicon, container, microbial diversity analysis

Requirements: Laptop with access to internet, a terminal software to log into our server such as Xshell (Windows and Mac) or Mobaxterm (Windows); data transfer software like FileZilla to transfer data from server to local computer; understand the basic microbiome concepts such as alpha and beta diversity although we will explain in the workshop.

Relevance: This workshop is designed for students and researchers who have interest in doing 16S amplicons microbiome analysis by themselves.

Dr Dr Xiaotao Jiang

Postdoctoral Research Fellow and Lead Bioinformatician, UNSW Microbiome Research Centre, St George & Sutherland Clinical School, Department of Medicine, UNSW Sydney

Dr Xiaotao Jiang is the Lead Bioinformatician and Postdoc Research Fellow at the Microbiome Research Centre (MRC), St George & Sutherland Clinical School,  the University of New South Wales, Sydney. He has over 10 years of experience in microbiome research using next/third-generation sequencing technologies. He completed his PhD at The University of Hong Kong studying wastewater microbiomes by investigating large-scale amplicon and shotgun metagenomic data sets. He also developed an antimicrobial resistance (AMR) profiling bioinformatics platform (ARGs OAP) to facilitate ARG quantification in metagenomic data set. Since joining UNSW MRC Dr Jiang lead the bioinformatics team working on numerous microbiome projects with multi-omics to understand the role of microbiome in human health and diseases.